Preparation and determination of polyclonal antibody to pre-mRNA splicing protein Tra2? / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2001.
Article
de Zh
| WPRIM
| ID: wpr-675150
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:
To prepare specific antibody against the pre mRNA splicing protein Tra2? in human fetal tissues.Methods:
A specific antigen fragment of human Tra2? was cloned into the pGEX 3x expression vector to express a recombinant GST Tra2? fusion protein.The fusion protein was expressed,purified and used as an antigen to immunize the New Zealand rabbit,and the polyclonal antibody to Tra2? was obtained.Results:
The antibody was successfully used to detect Tra2? protein in human fetal cerebrum with Western blot and immunohistochemistry analysis.Conclusion:
This prepard antibody has good specificity and can be used to detect the Tra2? protein in the human fetal tissues.
Texte intégral:
1
Base de données:
WPRIM
Langue:
Zh
Journal:
Chinese Journal of Immunology
Année:
2001
Type de document:
Article