THE EFFECTS OF LPS, HRIL — 2 AND PROTAMINE ON THE PROLIFERATION OF RAT GLONERULAR EPITHELIAL CELLS (GEC) AND INCORPORATION OF 35 SULFATE INTO CELL LAYERS IN VITRO / 中华肾脏病杂志

ABSTRACT

The heparan sulfate proteoglycan(HSPG) is the major part of sulfated macromolecules synthesized by the cultured rat GECs, and plays an important role in maintaining the glomerular charge—selective function. We have investigated the effects of lipopolysaccharide (LPS, 6.25ng/ml), human recombinant interleukin—2, (HrI1—2, 2. 5u/ml), and protamine sulfate(PtS, 100?g/ml) on the proliferation of rat GEC and incorporation of 35 SO into cell layers in vitro. A method of 3 H—TdR and 35 S—Na_2SO_4 dual isotope labelling technique was used. Statistical analysis was done using analysis of variance for both 3 H—TdR uptake and 35 SO incorporation, and analysis of variance only for the latter. These results show that LPS, HrIL—2 and PtS inhibited the proliferation of rat GECs. HrIL—2 also directly inhibited the incorporation of 35 SO into cell layers, but the roles of LPS and PtS, which decreased 35 SO—content, only came from their inhibition effects on cell proliferation. We suppose that the decreased production of HSPG by GECs, may contribute to the development of proteinuria.