Protective Effects of Danlou Tablet () against Murine Myocardial Ischemia and Reperfusion Injury In Vivo / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 613-620, 2018.
Article
de En
| WPRIM
| ID: wpr-691395
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the in vivo effect of Danlou Tablet (, DLT) on myocardial ischemia and reperfusion (I/R) injury.</p><p><b>METHODS</b>DLT effects were evaluated in mouse heart preparation using 30-min coronary occlusion followed by 24-h reperfusion and compared among sham group (n=6), I/R group (n=8), IPC group (ischemia preconditioning, n=6) and DLT group (I/R with DLT pretreatment for 3 days, 750 mg•kg•day, n=8). The effects of DLT were characterized in infarction size (IS) compared with risk region (RR) and left ventricle using the Evans blue/triphenyltetrazolium chloride double dye staining method in vivo. Furthermore, the dose-dependent effect of DLT on I/R injury was evaluated by double staining method. Five different concentrations of DLT (0.625, 1.25, 2.5, 5 and 10 g•kg•day) were chosen in this study, and dose-response curve of DLT was obtained on these data.</p><p><b>RESULTS</b>The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group (P<0.05 or P<0.01), the ratio of IS to RR was also reduced in the DLT and IPC groups (P<0.01), while there were no differences in RR among the four groups (P>0.05). Experiments showed incidence of arrhythmias was reduced in the DLT group (P<0.01). Furthermore, DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g•kg•day.</p><p><b>CONCLUSIONS</b>Our research concluded that DLT was effective in reducing I/R injury in mice, and provided experimental supports for the clinical use of DLT.</p>
Mots clés
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Anatomopathologie
/
Pharmacologie
/
Troubles du rythme cardiaque
/
Comprimés
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Température du corps
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Médicaments issus de plantes chinoises
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Cardiotoniques
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Lésion de reperfusion myocardique
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Facteurs de risque
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Utilisations thérapeutiques
Type d'étude:
Etiology_studies
/
Risk_factors_studies
Limites:
Animals
Langue:
En
Journal:
Chinese journal of integrative medicine
Année:
2018
Type de document:
Article