Establishment of protein expression profile of laser capture microdissection-purified nasopharyngeal carcinoma tissue / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 481-486, 2009.
Article
de Zh
| WPRIM
| ID: wpr-814300
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To establish the protein expression map of nasopharyngeal carcinoma (NPC), and provide a basis for proteomic study of NPC.@*METHODS@#Laser capture microdissection (LCM) was used to isolate cancer cells from NPC tissues. Two-dimensional gel electrophoresis(2-DE) was used to separate the total proteins of LCM purified NPC cells. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was performed to identify the proteins, and bioinformatics was used to construct the 2-DE database of NPC proteome.@*RESULTS@#A 2-DE reference map of NPC was established. On the 2-DE map, a total of (1 312+/-30) protein spots were detected, and 427 protein spots representing 241 non-redundant proteins were identified. The 2-DE database of NPC proteome was constructed. These data could be accessed at our website (http://www.xyproteomics.org).@*CONCLUSION@#A protein expression profile of LCM purified NPC tissues has been established for the first time, which provides useful information and source for proteomic study of NPC.
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Tumeurs du rhinopharynx
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Spectrométrie de masse MALDI
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Biologie informatique
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Protéome
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Analyse de profil d'expression de gènes
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Protéomique
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Microdissection
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Génétique
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Lasers
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Métabolisme
Limites:
Humans
Langue:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Année:
2009
Type de document:
Article