Pharmacokinetics and tissue distribution study in rats of asiaticoside-loaded modified liposomes / 中草药

ABSTRACT

Objective To prepare asiaticoside-loaded modified liposomes and to investigate the distribution and pharmacokinetics. Methods Different asiaticoside-loaded preparations (include solution, modified, and unmodified liposomes) were injected by tail vein in SD rats. HPLC method was used to detect the concentration of asiaticoside in the tissue and plasma samples. And the concentration-time profiles and pharmacokinetic parameters were then obtained and compared to get the variances. Results The concentration-time profiles of asiaticoside-loaded preparations guided along the single compartment model which the weight is 1/C2. The elimination half-life of asiaticoside solution and different asiaticoside liposomes were (14.52 ± 0.56), (101.35 ± 12.47), (149.82 ± 20.00), and (159.58 ± 16.46) min, respectively. The AUC of asiaticoside solution and different asiaticoside liposomes were (1 929.70 ± 159.00), (57 004.35 ± 8 710.89), (93 736.52 ± 12 710.76), and (64 737.48 ± 6 365.28) min∙μg/mL, respectively. The mass fraction of asiaticoside in each organ increased, especially in the pulmonary which increased from (4.94 ± 0.94) μg/g to (39.12 ± 12.04) μg/g. Conclusion The sustained release and targeting effects in SD rats were obvious of the asiaticoside-loaded modified liposomes.