Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesis / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 314-321, 2023.
Article
de En
| WPRIM
| ID: wpr-981945
Bibliothèque responsable:
WPRO
ABSTRACT
Mammalian testis exhibits remarkably high transcriptome complexity, and spermatogenesis undergoes two periods of transcriptional cessation. These make the RNA-binding proteins (RBPs) the utmost importance during male germ cell development. Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a large family of RBPs implicated in many steps of RNA processing; however, their roles in spermatogenesis are largely unknown. Here, we investigated the expression pattern of 12 hnRNP family members in mouse testes and found that most detected members are highly expressed in the testis. Furthermore, we found that most of the detected hnRNP proteins (hnRNPD, hnRNPK, hnRNPQ, hnRNPU, and hnRNPUL1) display the highest signals in the nuclei of pachytene spermatocytes, round spermatids, and Sertoli cells, whereas hnRNPE1 exclusively concentrates in the manchette of elongating spermatids. The expression of these hnRNP proteins showed both similarities and specificity, suggesting their diverse roles in spermatogenesis.
Mots clés
Texte intégral:
1
Base de données:
WPRIM
Sujet principal:
Cellules de Sertoli
/
Spermatides
/
Spermatocytes
/
Spermatogenèse
/
Testicule
/
Protéines de liaison à l'ARN
/
Ribonucléoprotéines nucléaires hétérogènes
/
Mammifères
Limites:
Animals
Langue:
En
Journal:
Asian Journal of Andrology
Année:
2023
Type de document:
Article