Yeast HXK2 gene reverts glucose regulation mutation of penicillin biosynthesis in P. chrysogenum
Braz. j. microbiol
; Braz. j. microbiol;45(3): 873-883, July-Sept. 2014. ilus, graf, tab
Article
in En
| LILACS
| ID: lil-727016
Responsible library:
BR1.1
ABSTRACT
The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in different degrees, and acquired a hexokinase (fructose phosphorylating) activity absent in strains AS- P-78 and dogR5. Interestingly, they also recovered glucose regulation of β-galactosidase. On the other hand, glucokinase activity was affected in different ways in the transformants; one of which showed a lower activity than the parental dogR5, but normal glucose regulation of penicillin biosynthesis. Our results show that Penicillium chrysogenum AS-P-78 and dogR5 strains lack hexokinase, and suggest that an enzyme with glucokinase activity is involved in glucose regulation of penicillin biosynthesis and β-galactosidase, thus signaling glucose in both primary and secondary metabolism; however, catalytic and signaling activities seem to be independent.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Penicillins
/
Penicillium chrysogenum
/
Gene Expression Regulation, Fungal
/
Saccharomyces cerevisiae Proteins
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Glucose
/
Hexokinase
Language:
En
Journal:
Braz J Microbiol
/
Braz. j. microbiol
/
Braz. j. microbiol. (Online)
/
Brazilian journal of microbiology (Impresso)
Journal subject:
MICROBIOLOGIA
Year:
2014
Document type:
Article
Affiliation country:
Country of publication: