Liquid chromatography-fast atom bombardment mass spectrometry for detection and determination of pentazocine in human tissues.
J Chromatogr B Biomed Sci Appl
; 731(1): 149-54, 1999 Aug 06.
Article
in En
| MEDLINE
| ID: mdl-10492001
A reliable and sensitive method was developed for the detection and determination of pentazocine in human solid tissues using liquid chromatography-dynamic fast atom bombardment (FAB) mass spectrometry, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as an internal standard. The extract was evaporated to dryness and dissolved in the mobile phase, acetonitrile-10 mM ammonium acetate solution (20:80, pH 4.0) containing 0.5% glycerol as FAB matrix. The eluent was pumped at a flow rate of 25 microl/min and split before introduction to FAB mass spectrometer. Quantitative analysis was carried out by means of monitoring quasi-molecular ions with m/z 286 for pentazocine and m/z 284 for levallorphan. The lower limit of detection of pentazocine in each tissue tested was 1 ng/g with scan mode and 0.1 ng/g with SIM mode. Using this method, the concentrations of pentazocine were determined in the tissues of an autopsied individual to perform toxicological evaluation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pentazocine
/
Chromatography, Liquid
/
Spectrometry, Mass, Fast Atom Bombardment
Type of study:
Diagnostic_studies
Limits:
Aged
/
Female
/
Humans
Language:
En
Journal:
J Chromatogr B Biomed Sci Appl
Journal subject:
QUIMICA CLINICA
Year:
1999
Document type:
Article
Affiliation country:
Country of publication: