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Liquid chromatography-fast atom bombardment mass spectrometry for detection and determination of pentazocine in human tissues.
Imamura, T; Kudo, K; Namera, A; Tokunaga, H; Yashiki, M; Jitsufuchi, N; Kojima, T.
Affiliation
  • Imamura T; Department of Legal Medicine, Hiroshima University School of Medicine, Japan. tohrui@ipc.hiroshima-u.ac.jp
J Chromatogr B Biomed Sci Appl ; 731(1): 149-54, 1999 Aug 06.
Article in En | MEDLINE | ID: mdl-10492001
A reliable and sensitive method was developed for the detection and determination of pentazocine in human solid tissues using liquid chromatography-dynamic fast atom bombardment (FAB) mass spectrometry, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as an internal standard. The extract was evaporated to dryness and dissolved in the mobile phase, acetonitrile-10 mM ammonium acetate solution (20:80, pH 4.0) containing 0.5% glycerol as FAB matrix. The eluent was pumped at a flow rate of 25 microl/min and split before introduction to FAB mass spectrometer. Quantitative analysis was carried out by means of monitoring quasi-molecular ions with m/z 286 for pentazocine and m/z 284 for levallorphan. The lower limit of detection of pentazocine in each tissue tested was 1 ng/g with scan mode and 0.1 ng/g with SIM mode. Using this method, the concentrations of pentazocine were determined in the tissues of an autopsied individual to perform toxicological evaluation.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Pentazocine / Chromatography, Liquid / Spectrometry, Mass, Fast Atom Bombardment Type of study: Diagnostic_studies Limits: Aged / Female / Humans Language: En Journal: J Chromatogr B Biomed Sci Appl Journal subject: QUIMICA CLINICA Year: 1999 Document type: Article Affiliation country: Country of publication:
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Collection: 01-internacional Database: MEDLINE Main subject: Pentazocine / Chromatography, Liquid / Spectrometry, Mass, Fast Atom Bombardment Type of study: Diagnostic_studies Limits: Aged / Female / Humans Language: En Journal: J Chromatogr B Biomed Sci Appl Journal subject: QUIMICA CLINICA Year: 1999 Document type: Article Affiliation country: Country of publication: