Cryo-techniques applied to stratum corneum with description of a new sample holder for cryo-scanning electron microscopy of freeze-fractured samples.

ABSTRACT

Stratum corneum structure greatly differs from that of the living epidermis and specific sample cryo-preparation techniques have to be used. Practical aspects of these cryo-techniques applied to stratum corneum are discussed. Emphasis is placed on scanning electron microscopy of cryo-fixed samples. A new sample holder designed for cryo-scanning electron microscopy of freeze-fractured stratum corneum is described.