Varying results for immunoassay screening kits for cotinine level.
Psychol Addict Behav
; 16(3): 256-9, 2002 Sep.
Article
in En
| MEDLINE
| ID: mdl-12236461
ABSTRACT
Our earlier study found that although enzyme-linked immunosorbent analysis (ELISA) screening assays for urine cotinine indicated use in former smoking treatment patients who reported abstinence, this finding was sometimes incorrect when validated against gas chromatography/mass spectrometry (GC/ MS; P. Gariti, A. I. Alterman, R. Ehrmann, F. D. Mulvaney, & C. P. O'Brien, 2002). In the current validation study, separate urine samples of 71 of these same patients were reanalyzed by an independent laboratory in blinded fashion using a screening enzyme immunoassay (EIA) analysis and GC/MS confirmation. EIA results showed almost total agreement with confirmatory testing. The findings indicate that use of screening ELISA/EIA for urine cotinine can detect unreported cases of smoking in former patients, but that care is needed in selecting a laboratory for conducting these tests.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reagent Kits, Diagnostic
/
Substance Abuse Detection
/
Immunoenzyme Techniques
/
Smoking Cessation
/
Cotinine
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Screening_studies
Limits:
Humans
Language:
En
Journal:
Psychol Addict Behav
Journal subject:
PSICOLOGIA
/
TRANSTORNOS RELACIONADOS COM SUBSTANCIAS
Year:
2002
Document type:
Article
Affiliation country: