A fluorogenic dye activated by the staudinger ligation.
J Am Chem Soc
; 125(16): 4708-9, 2003 Apr 23.
Article
in En
| MEDLINE
| ID: mdl-12696879
Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphines
/
Azides
/
Coumarins
/
Fluorescent Dyes
Limits:
Animals
Language:
En
Journal:
J Am Chem Soc
Year:
2003
Document type:
Article
Affiliation country:
Country of publication: