Identification of 20 microRNAs from Oryza sativa.
Nucleic Acids Res
; 32(5): 1688-95, 2004.
Article
in En
| MEDLINE
| ID: mdl-15020705
ABSTRACT
MicroRNAs (miRNAs) are present in both plant and animal kingdoms and represents a growing family of non-coding RNAs. These tiny RNAs act as small guides and direct negative regulations usually in the process of development through sequence complementarity to target mRNAs. Although a large number of miRNAs have been identified from various animals, so far plant miRNA studies have focused mainly on Arabidopsis. Here we describe the identification of 20 miRNAs from a rice cDNA library. All the miRNAs were presumably processed from precursors with stem-loop structures and were positively detected in rice cells from at least one tissue, some of which showed tissue-specific expression. Twenty-three unique rice genes were identified to be feasible targets for seven rice miRNAs, including four members of Scarecrow-like transcription factor, the targets of miR-39 that had been characterized in Arabidopsis. Lacking long complementarity, the regulatory targets of 13 miRNAs remain to be further investigated. A possible mechanism of translational repressor for plant miRNAs that lack perfect complementarity to target mRNAs is discussed.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oryza
/
RNA, Plant
/
MicroRNAs
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
Nucleic Acids Res
Year:
2004
Document type:
Article