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Use of enzyme-linked immunosorbent assay for detection of natural leishmania infections in phlebotomine sand flies from southeastern Turkey.
Pazarbasi, Ayfer; Alptekin, Davut; Luleyap, H Umit; Kasap, Mulkiye; Kasap, Halil.
Affiliation
  • Pazarbasi A; Department of Medical Biology and Genetics, School of Medicine, University of Cukurova, 01330 Balcali, Adana, Turkey.
J Med Entomol ; 43(2): 248-51, 2006 Mar.
Article in En | MEDLINE | ID: mdl-16619606
In total, 320 phelebotomine sand flies from Alibozlu (Osmaniye), Kizyusuflu (Osmaniye), and Sanliurfa in southeastern Turkey were tested for the detection and identification of Leishmania in vector sand flies by enzyme-linked immunosorbent assay with species-specific monoclonal antibodies. We used monoclonal antibodies that recognize both Leishmania tropica and Leishmania major, and a monoclonal antibody specific only to L. tropica. Phosphate-buffered saline and monoclonal antibody M2 recognizing Leishmania amazonensis were used as controls. Infection rates of sand flies were 0.9% in Alibozlu, 0% Kizyusuflu, and 3.6% in Sanliurfa. Positive sand flies were identified as Phlebotomus sergenti Parrot and Phlebotomus major syriacus Adler & Theodor.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Phlebotomus / Insect Vectors / Leishmania Type of study: Diagnostic_studies / Prevalence_studies / Risk_factors_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: J Med Entomol Year: 2006 Document type: Article Affiliation country: Country of publication:
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Collection: 01-internacional Database: MEDLINE Main subject: Phlebotomus / Insect Vectors / Leishmania Type of study: Diagnostic_studies / Prevalence_studies / Risk_factors_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: J Med Entomol Year: 2006 Document type: Article Affiliation country: Country of publication: