Temporal and spatial delineation of mouse Otx2 functions by conditional self-knockout.
EMBO Rep
; 7(8): 824-30, 2006 Aug.
Article
in En
| MEDLINE
| ID: mdl-16845372
ABSTRACT
To identify the independent spatial and temporal activities of the essential developmental gene the Otx2, the germline mutation of which is lethal at embryonic day 8.5, we floxed one allele and substituted the other with an inducible CreER recombinase gene. This makes 'trans' self-knockout possible at any developmental stage. The transient action of tamoxifen pulses allows time-course mutation. We demonstrate efficient temporal knockout and demarcate spatio-temporal windows in which Otx2 controls the head, brain structures and body development.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression Regulation, Developmental
/
Otx Transcription Factors
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
EMBO Rep
Journal subject:
BIOLOGIA MOLECULAR
Year:
2006
Document type:
Article
Affiliation country: