Development of an immune microanalysis system by use of peroxyoxalate chemiluminescence detection.
Anal Sci
; 23(6): 739-41, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-17575360
ABSTRACT
We developed an immune microanalysis system incorporating chemiluminescence detection, where the peroxyoxalate chemiluminescence (CL) detection using bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl]oxalate (TDPO)-hydrogen peroxide (H2O2)-fluorescein isothiocianate (FITC) reaction was newly adopted. The analysis system performed the following three processes on a microchip immune reaction for high selectivity, electrophoresis for formation and transportation of the sample plug, and CL detection. The immune reaction was carried out using an antibody-immobilized glass bead. The glass bead was placed in one of the reservoirs in the microchip along with antigen (analyte) and a known amount of FITC-labeled antigen to set up a competitive immune reaction. The reactant after the immune reaction was fed electrophoretically into the intersection, resulting in a sample plug. The sample plug was then moved into another reservoir containing TDPO-H2O2 acetonitrile solution. At this point, CL detection was performed. The system described here was capable of determining human serum albumin or immunosuppressive acidic protein as a cancer marker in human serum.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxalates
/
Proteins
/
Microchip Analytical Procedures
/
Luminescent Measurements
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Anal Sci
Year:
2007
Document type:
Article
Affiliation country: