Phage immobilized magnetoelastic sensor for the detection of Salmonella typhimurium.
J Microbiol Methods
; 71(1): 55-60, 2007 Oct.
Article
in En
| MEDLINE
| ID: mdl-17765344
ABSTRACT
In this article, a phage-based magnetoelastic sensor for the detection of Salmonella typhimurium is reported. Filamentous bacteriophage specific to S. typhimurium was used as a biorecognition element in order to ensure specific and selective binding of bacteria onto the sensor surface. Phage was immobilized onto the surface of the sensors by physical adsorption. The phage immobilized magnetoelastic sensors were exposed to S. typhimurium cultures with different concentrations ranging from 5x10(1) to 5x10(8) cfu/ml, and the corresponding changes in resonance frequency response of the sensor were studied. It was experimentally established that the sensitivity of the magnetoelastic sensors was higher for sensors with smaller physical dimensions. An increase in sensitivity from 159 Hz/decade for a 2 mm sensor to 770 Hz/decade for a 1 mm sensor was observed. Scanning electron microscopy (SEM) analysis of previously assayed biosensors provided visual verification of frequency changes that were caused by S. typhimurium binding to phage immobilized on the sensor surface. The detection limit on the order of 10(3) cfu/ml was obtained for a sensor with dimensions 1x0.2x0.015 mm.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Salmonella Infections
/
Salmonella typhimurium
/
Bacteriophages
/
Biosensing Techniques
/
Magnetics
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Microbiol Methods
Year:
2007
Document type:
Article
Affiliation country: