Your browser doesn't support javascript.
loading
In situ proteolysis for protein crystallization and structure determination.
Dong, Aiping; Xu, Xiaohui; Edwards, Aled M; Chang, Changsoo; Chruszcz, Maksymilian; Cuff, Marianne; Cymborowski, Marcin; Di Leo, Rosa; Egorova, Olga; Evdokimova, Elena; Filippova, Ekaterina; Gu, Jun; Guthrie, Jennifer; Ignatchenko, Alexandr; Joachimiak, Andrzej; Klostermann, Natalie; Kim, Youngchang; Korniyenko, Yuri; Minor, Wladek; Que, Qiuni; Savchenko, Alexei; Skarina, Tatiana; Tan, Kemin; Yakunin, Alexander; Yee, Adelinda; Yim, Veronica; Zhang, Rongguang; Zheng, Hong; Akutsu, Masato; Arrowsmith, Cheryl; Avvakumov, George V; Bochkarev, Alexey; Dahlgren, Lars-Göran; Dhe-Paganon, Sirano; Dimov, Slav; Dombrovski, Ludmila; Finerty, Patrick; Flodin, Susanne; Flores, Alex; Gräslund, Susanne; Hammerström, Martin; Herman, Maria Dolores; Hong, Bum-Soo; Hui, Raymond; Johansson, Ida; Liu, Yongson; Nilsson, Martina; Nedyalkova, Lyudmila; Nordlund, Pär; Nyman, Tomas.
Affiliation
  • Dong A; Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada.
Nat Methods ; 4(12): 1019-21, 2007 Dec.
Article in En | MEDLINE | ID: mdl-17982461
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Hydrolases / Proteins / Crystallization / Crystallography Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2007 Document type: Article Affiliation country: Country of publication:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Hydrolases / Proteins / Crystallization / Crystallography Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2007 Document type: Article Affiliation country: Country of publication: