In situ proteolysis for protein crystallization and structure determination.
Nat Methods
; 4(12): 1019-21, 2007 Dec.
Article
in En
| MEDLINE
| ID: mdl-17982461
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Hydrolases
/
Proteins
/
Crystallization
/
Crystallography
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2007
Document type:
Article
Affiliation country:
Country of publication: