[High-performance liquid chromatography-mass spectrometry for determining olmesartan in human plasma].
Nan Fang Yi Ke Da Xue Xue Bao
; 28(6): 1104-5, 2008 Jun.
Article
in Zh
| MEDLINE
| ID: mdl-18583274
ABSTRACT
OBJECTIVE:
To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.METHODS:
Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(146026) with a flow rate of 0.22 ml/min.RESULTS:
The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.CONCLUSION:
The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tetrazoles
/
Imidazoles
Limits:
Humans
Language:
Zh
Journal:
Nan Fang Yi Ke Da Xue Xue Bao
Year:
2008
Document type:
Article
Affiliation country: