[Studies of betuionic acid on cell cycle and related protein expressions on mice of bearing H22 tumor cells].

ABSTRACT

OBJECTIVE: To study the betulonic acid on the cell cycle and related protein expressions on mice of bearing H22 tumor cells. METHOD: Flow cytometray was used to observe the changes of betulonic acid on the cell cycle and P53 of H22 tumor cells. Immunohistochemistry was determined the espressions of PI3K and AKT. RESULT: Increasing the doses of betulonic acid, the number of H22 cells in S phase and G2 phase was increasing gradually, it can speculate that when the betulonic acid act on cells, the cells were blocked in S and G2 phase and inhibited the protein expressions of PI3K and AKT. CONCLUSION: Betulonic acid may be up-regulate the activity of P53 and inhibite the expressions of PI3K and AKT, so that it inhibited the survival pathway of tumor cells.