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Detection of cytochrome P450-2A6, -3A5 and -4B1 with real-time polymerase chain reaction in prostate tissue.
Zencir, Sevil; Alptekin, Davut; Celiktas, Medih; Canturk, Pakize; Colak, Deniz; Caner, Vildan; Luleyap, Umit H; Topcu, Zeki.
Affiliation
  • Zencir S; Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Z Naturforsch C J Biosci ; 63(9-10): 780-4, 2008.
Article in En | MEDLINE | ID: mdl-19040121
Cytochrome P450 (CYP) is a heme-containing enzyme superfamily metabolizing a wide variety of xenobiotics, including drugs and carcinogens. The majority of CYP genes are expressed in the liver, however, some CYP isoforms are also reported for a number of extra hepatic tissues. We analyzed Cytochrome P450-2A6, -3A5 and -4B1 mRNAs using real-time reverse-transcriptase polymerase chain reaction (RT-PCR) in a total of 21 homogenized prostate tissues with or without malignancy. We detected a consistent expression of CYP2A6 and CYP3A5 in all, and of CYP4B1 in some (11/21) of the samples at mRNA level. Neither the histopathological status nor the smoking habit of the individuals affected CYP4B1 expression. Our results reflect possible roles for these particular CYPs in therapy and protection of prostate tissue.
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Collection: 01-internacional Database: MEDLINE Main subject: Prostate / RNA, Messenger / Aryl Hydrocarbon Hydroxylases / Cytochrome P-450 CYP3A Type of study: Diagnostic_studies Limits: Humans / Male Language: En Journal: Z Naturforsch C J Biosci Year: 2008 Document type: Article Affiliation country: Country of publication:
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Collection: 01-internacional Database: MEDLINE Main subject: Prostate / RNA, Messenger / Aryl Hydrocarbon Hydroxylases / Cytochrome P-450 CYP3A Type of study: Diagnostic_studies Limits: Humans / Male Language: En Journal: Z Naturforsch C J Biosci Year: 2008 Document type: Article Affiliation country: Country of publication: