DNA content of hepatocyte and erythrocyte nuclei of the spined loach (Cobitis taenia L.) and its polyploid forms.
Fish Physiol Biochem
; 36(3): 523-529, 2010 Sep.
Article
in En
| MEDLINE
| ID: mdl-19377846
ABSTRACT
We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taenia (diploid) and its allopolyploid forms. Twenty triploid females and one tetraploid were used. At least 20,000 hepatocyte and erythrocyte nuclei were acquired and analyzed by flow cytometry. C. taenia erythrocyte nuclei contain 3.15 +/- 0.21 pg of DNA and the hepatocyte nuclei 4.45 +/- 0.46 pg of DNA. Triploid Cobitis have 5.08 +/- 0.41 pg of DNA in erythrocyte nuclei and 6.11 +/- 0.40 pg of DNA in hepatocyte nuclei, whereas the tetraploid erythrocyte and hepatocyte nuclei contained 6.60 and 7.40 pg of DNA, respectively. In general, the DNA contents correlate positively with the ploidy level of the fish investigated. The DNA content variation in the hepatocyte and erythrocyte nuclei may be due to differences in extent of chromatin condensation, which is more pronounced in the erythrocyte than hepatocyte nuclei, or to the several orders of ploidy that occur in the parenchymal liver cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyploidy
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Cypriniformes
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DNA
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Cell Nucleus
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Hepatocytes
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Erythrocytes
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Fish Physiol Biochem
Year:
2010
Document type:
Article
Affiliation country: