Downregulation of miR-21 enhances chemotherapeutic effect of taxol in breast carcinoma cells.
Technol Cancer Res Treat
; 9(1): 77-86, 2010 Feb.
Article
in En
| MEDLINE
| ID: mdl-20082533
ABSTRACT
The successful of anti-cancer treatment are often limited by the development of drug resistance. Recent work has highlighted the involvement of non-coding RNAs, microRNAs(miRNAs) in cancer development, and their possible involvement in the evolution of drug resistance has been proposed. In this study, we combine taxol chemotherapy and miR-21 inhibitor treatment via polyamidoamine (PAMAM) dendrimers vector to evaluate the effects of combination therapy on suppression of breast cancer cells. The 50% inhibitory concentration (IC50) values for taxol were significantly decreased to a greater extent in the cells transfected with miR-21 inhibitor compared with cells treated with taxol alone. Taxol treatment also increased the percentage of apoptotic breast cancer cells in miR-21 inhibitor transfected cells compared with control cells. Furthermore, treatment of the miR-21 inhibitor-transfected cells with the anti-cancer drugs taxol resulted in significantly reduced cell viability and invasiveness compared with control cells. These results indicated that the miR-21 plays an important role in the resistance of breast carcinoma cells to chemotherapeutic drugs. Therefore, miR-21 inhibitor gene therapy combined with taxol chemotherapy might represent a promising novel therapeutic approach for the treatment of breast malignancies.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breast Neoplasms
/
Genetic Therapy
/
Adenocarcinoma
/
Paclitaxel
/
MicroRNAs
/
Dendrimers
/
Antineoplastic Agents, Phytogenic
Limits:
Female
/
Humans
Language:
En
Journal:
Technol Cancer Res Treat
Journal subject:
NEOPLASIAS
/
TERAPEUTICA
Year:
2010
Document type:
Article