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Diacylglycerol kinase alpha mediates HGF-induced Rac activation and membrane ruffling by regulating atypical PKC and RhoGDI.
Chianale, Federica; Rainero, Elena; Cianflone, Cristina; Bettio, Valentina; Pighini, Andrea; Porporato, Paolo E; Filigheddu, Nicoletta; Serini, Guido; Sinigaglia, Fabiola; Baldanzi, Gianluca; Graziani, Andrea.
Affiliation
  • Chianale F; Department of Clinical and Experimental Medicine and Biotecnologie per la Ricerca Medica Applicata, University Amedeo Avogadro of Piemonte Orientale, 28100 Novara, Italy.
Proc Natl Acad Sci U S A ; 107(9): 4182-7, 2010 Mar 02.
Article in En | MEDLINE | ID: mdl-20160093
Diacylglycerol kinases (DGKs) convert diacylglycerol (DAG) into phosphatidic acid (PA), acting as molecular switches between DAG- and PA-mediated signaling. We previously showed that Src-dependent activation and plasma membrane recruitment of DGKalpha are required for growth-factor-induced cell migration and ruffling, through the control of Rac small-GTPase activation and plasma membrane localization. Herein we unveil a signaling pathway through which DGKalpha coordinates the localization of Rac. We show that upon hepatocyte growth-factor stimulation, DGKalpha, by producing PA, provides a key signal to recruit atypical PKCzeta/iota (aPKCzeta/iota) in complex with RhoGDI and Rac at ruffling sites of colony-growing epithelial cells. Then, DGKalpha-dependent activation of aPKCzeta/iota mediates the release of Rac from the inhibitory complex with RhoGDI, allowing its activation and leading to formation of membrane ruffles, which constitute essential requirements for cell migration. These findings highlight DGKalpha as the central element of a lipid signaling pathway linking tyrosine kinase growth-factor receptors to regulation of aPKCs and RhoGDI, and providing a positional signal regulating Rac association to the plasma membrane.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Kinase C / Hepatocyte Growth Factor / Diacylglycerol Kinase / Rac GTP-Binding Proteins / Guanine Nucleotide Dissociation Inhibitors Limits: Animals Language: En Journal: Proc Natl Acad Sci U S A Year: 2010 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Kinase C / Hepatocyte Growth Factor / Diacylglycerol Kinase / Rac GTP-Binding Proteins / Guanine Nucleotide Dissociation Inhibitors Limits: Animals Language: En Journal: Proc Natl Acad Sci U S A Year: 2010 Document type: Article Affiliation country: Country of publication: