Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice.
EMBO Rep
; 11(6): 445-51, 2010 Jun.
Article
in En
| MEDLINE
| ID: mdl-20467437
ABSTRACT
Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 5' and 3' alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nuclear Proteins
/
RNA-Binding Proteins
/
Cell Cycle Proteins
/
RNA Splice Sites
/
Heterogeneous-Nuclear Ribonucleoprotein Group M
/
Intracellular Signaling Peptides and Proteins
Limits:
Humans
Language:
En
Journal:
EMBO Rep
Journal subject:
BIOLOGIA MOLECULAR
Year:
2010
Document type:
Article
Affiliation country: