Identification of new minor metabolites of penicillin G in human serum by multiple-stage tandem mass spectrometry.
Rapid Commun Mass Spectrom
; 25(1): 25-32, 2011 Jan 15.
Article
in En
| MEDLINE
| ID: mdl-21154651
ABSTRACT
Liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) were applied to characterize drug metabolites. Although these two methods have overcome the identification and structural characterization of metabolites analysis, they remain time-consuming processes. In this study, a novel multiple-stage tandem mass spectrometric method (MS(n) ) was evaluated for identification and characterization of new minor metabolism profiling of penicillin G, one of the ß-lactam antibiotics, in human serum. Seven minor metabolites including five phase I metabolites and two phase II metabolites of penicillin G were identified by using data-dependent LC/MS(n) screening in one chromatographic run. The accuracy masses of seven identified metabolites of penicillin G were also confirmed by mass spectral calibration software (MassWorks™). The proposed data-dependent LC/MS(n) method is a powerful tool to provide large amounts of the necessary structural information to characterize minor metabolite in metabolism profiling.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Penicillin G
/
Tandem Mass Spectrometry
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2011
Document type:
Article