Peptide reactivity between multiple sclerosis (MS) CSF IgG and recombinant antibodies generated from clonally expanded plasma cells in MS CSF.
J Neuroimmunol
; 233(1-2): 192-203, 2011 Apr.
Article
in En
| MEDLINE
| ID: mdl-21176973
ABSTRACT
We employed 19 recombinant antibodies (rAbs) generated from clonally expanded plasma cells, and native IgG from cerebrospinal fluid (CSF) of three multiple sclerosis (MS) patients for panning with phage displayed random peptide libraries. Specific peptide epitopes/mimotopes were identified and characterized. Importantly, peptide-antibody interactions were shared by rAbs and native IgG from the same patient. Three peptides strongly interacted with at least one other MS CSF, but not to inflammatory CNS controls. Database searches revealed several protein candidates including stress proteins, cell surface proteins, and neuronal proteins. Peptides derived from the candidate proteins were recognized by rAbs. Identification of peptide epitopes/mimotopes in MS may provide clues regarding disease-relevant antigens.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Fragments
/
Plasma Cells
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Autoantibodies
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Recombinant Proteins
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Immunoglobulin G
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Multiple Sclerosis
Limits:
Humans
Language:
En
Journal:
J Neuroimmunol
Year:
2011
Document type:
Article
Affiliation country: