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A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries.
Fisher, Sheila; Barry, Andrew; Abreu, Justin; Minie, Brian; Nolan, Jillian; Delorey, Toni M; Young, Geneva; Fennell, Timothy J; Allen, Alexander; Ambrogio, Lauren; Berlin, Aaron M; Blumenstiel, Brendan; Cibulskis, Kristian; Friedrich, Dennis; Johnson, Ryan; Juhn, Frank; Reilly, Brian; Shammas, Ramy; Stalker, John; Sykes, Sean M; Thompson, Jon; Walsh, John; Zimmer, Andrew; Zwirko, Zac; Gabriel, Stacey; Nicol, Robert; Nusbaum, Chad.
Affiliation
  • Fisher S; Genome Sequencing Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02141, USA.
Genome Biol ; 12(1): R1, 2011.
Article in En | MEDLINE | ID: mdl-21205303
ABSTRACT
Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Library / Oligonucleotide Array Sequence Analysis / Automation, Laboratory / Exome / Nucleic Acid Hybridization Limits: Humans Language: En Journal: Genome Biol Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2011 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Library / Oligonucleotide Array Sequence Analysis / Automation, Laboratory / Exome / Nucleic Acid Hybridization Limits: Humans Language: En Journal: Genome Biol Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2011 Document type: Article Affiliation country: