[Construction of sub-unit dengue vaccines and analysis of its immunogenicity].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 24(6): 430-2, 2010 Dec.
Article
in Zh
| MEDLINE
| ID: mdl-21604567
ABSTRACT
OBJECTIVE:
To construct sub-unit vaccines of dengue virus type 1 to 4 and to analyze its immunogenicity.METHODS:
Envelope domain III s of dengue serotypes 1 and 2, as well as 3 and 4, were spliced by a linker (Gly-Gly-Ser-Gly-Ser)3 and cloned into vector pET-30a, then transformed into E. coli to express recombinant fusion proteins. The recombinant proteins were purified by high-performance liquid chromatography and mixed to immunize BALB/c mice. The neutralizing antibodies were tested by neutralizing assay, as well as in newborn mice challenged intracranially with dengue virus type 1 to 4.RESULTS:
Mice immunized with proteins could produce neutralizing antibodies, with titers of 134. 9, 1 45.3, 1 24.7 and 138.4 for DEN-1 to 4 respectively. 100% newborn mice challenged with DEN-1 or 2 in combination with sera from mice immunized with recombinant proteins were protected, whereas 83% protection was obtained when challenged with DEN-3 or 4.CONCLUSION:
The recombinant proteins possess excellent immunogenicity to induce neutralizing antibodies and would be valuable for development of a tetravalent sub-unit vaccine.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Envelope Proteins
/
Dengue Virus
/
Dengue Vaccines
Limits:
Animals
Language:
Zh
Journal:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Journal subject:
VIROLOGIA
Year:
2010
Document type:
Article
Affiliation country: