Occurrence of two different species of mitoviruses in the European race of Gremmeniella abietina var. abietina, both hosted by the genetically unique Spanish population.
Fungal Biol
; 116(8): 872-82, 2012 Aug.
Article
in En
| MEDLINE
| ID: mdl-22862915
ABSTRACT
The genetic structure of the genus Mitovirus community hosted by the European pathogenic conifer fungus Gremmeniella abietina var. abietina was investigated. Gremmeniella abietina is a species complex with a divergent mycovirus community, composed mainly of Totivirus, Partitivirus, and Mitovirus species. In this work, the total doubled-stranded (ds)RNA from 353 isolates from Canada, Finland, Spain, Switzerland, Turkey, and USA was extracted to look for the presence of a ca. 2.5 kb band typical of mitoviruses' genomes. Based on the banding data, 60 partial RNA-dependent RNA polymerase (RdRp) DNA sequences (ca. 500 bp) were amplified with reverse transcription-polymerase chain reaction (RT-PCR) and sequenced. Two distantly related mitovirus groups (species) were observed in the clustering analysis, one of them related to GMV1-1 and the other one related to a new putative species described in this study, GMV2-1. Viruses in these two clusters seemed to be subjected to purifying selection. The cluster with GMV1-1 included viruses observed in the Finnish biotype A and Spanish strains, whereas the cluster including GMV2-1 was composed of viruses of the Finnish biotype B and one from the Spanish population. Thereby, the Spanish population of G. abietina harboured mitovirus strains occurring in both biotype A and B strains, and it is the first one hosting distantly related mycoviruses of a single genus in one population of G. abietina. This may suggest that horizontal transmission of viruses could have occurred between biotype B and the Spanish population.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ascomycota
/
RNA Viruses
Aspects:
Determinantes_sociais_saude
Country/Region as subject:
Europa
Language:
En
Journal:
Fungal Biol
Journal subject:
MICROBIOLOGIA
Year:
2012
Document type:
Article
Affiliation country: