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Gene loops enhance transcriptional directionality.
Tan-Wong, Sue Mei; Zaugg, Judith B; Camblong, Jurgi; Xu, Zhenyu; Zhang, David W; Mischo, Hannah E; Ansari, Aseem Z; Luscombe, Nicholas M; Steinmetz, Lars M; Proudfoot, Nick J.
Affiliation
  • Tan-Wong SM; Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Science ; 338(6107): 671-5, 2012 Nov 02.
Article in En | MEDLINE | ID: mdl-23019609
Eukaryotic genomes are extensively transcribed, forming both messenger RNAs (mRNAs) and noncoding RNAs (ncRNAs). ncRNAs made by RNA polymerase II often initiate from bidirectional promoters (nucleosome-depleted chromatin) that synthesize mRNA and ncRNA in opposite directions. We demonstrate that, by adopting a gene-loop conformation, actively transcribed mRNA encoding genes restrict divergent transcription of ncRNAs. Because gene-loop formation depends on a protein factor (Ssu72) that coassociates with both the promoter and the terminator, the inactivation of Ssu72 leads to increased synthesis of promoter-associated divergent ncRNAs, referred to as Ssu72-restricted transcripts (SRTs). Similarly, inactivation of individual gene loops by gene mutation enhances SRT synthesis. We demonstrate that gene-loop conformation enforces transcriptional directionality on otherwise bidirectional promoters.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Transcription, Genetic / RNA, Messenger / RNA, Untranslated / Genes, Fungal Language: En Journal: Science Year: 2012 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Transcription, Genetic / RNA, Messenger / RNA, Untranslated / Genes, Fungal Language: En Journal: Science Year: 2012 Document type: Article Country of publication: