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Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR.
van Dommelen, Laura; Wolffs, Petra F G; van Tiel, Frank H; Dukers, Nicole; Herngreen, Selma B; Bruggeman, Cathrien A; Hoebe, Christian J P A.
Affiliation
  • van Dommelen L; Department of Medical Microbiology, Maastricht University Medical Centre, Maastricht, The Netherlands.
J Clin Microbiol ; 51(3): 990-2, 2013 Mar.
Article in En | MEDLINE | ID: mdl-23254134
We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / DNA, Bacterial / Chlamydia trachomatis / Bacteriological Techniques / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies / Evaluation_studies Limits: Female / Humans Language: En Journal: J Clin Microbiol Year: 2013 Document type: Article Affiliation country: Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / DNA, Bacterial / Chlamydia trachomatis / Bacteriological Techniques / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies / Evaluation_studies Limits: Female / Humans Language: En Journal: J Clin Microbiol Year: 2013 Document type: Article Affiliation country: Country of publication: