Expression, purification and crystallization of the ancestral androgen receptor-DHT complex.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 69(Pt 9): 994-6, 2013 Sep.
Article
in En
| MEDLINE
| ID: mdl-23989146
ABSTRACT
Steroid receptors (SRs) are a closely related family of ligand-dependent nuclear receptors that mediate the transcription of genes critical for development, reproduction and immunity. SR dysregulation has been implicated in cancer, inflammatory diseases and metabolic disorders. SRs bind their cognate hormone ligand with exquisite specificity, offering a unique system to study the evolution of molecular recognition. The SR family evolved from an estrogen-sensitive ancestor and diverged to become sensitive to progestagens, corticoids and, most recently, androgens. To understand the structural mechanisms driving the evolution of androgen responsiveness, the ancestral androgen receptor (ancAR1) was crystallized in complex with 5α-dihydrotestosterone (DHT) and a fragment of the transcriptional mediator/intermediary factor 2 (Tif2). Crystals diffracted to 2.1â
Å resolution and the resulting structure will permit a direct comparison with its progestagen-sensitive ancestor, ancestral steroid receptor 2 (AncSR2).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dihydrotestosterone
/
Receptors, Androgen
/
Nuclear Receptor Coactivator 2
Limits:
Humans
Language:
En
Journal:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Year:
2013
Document type:
Article
Affiliation country: