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A new, rapid and sensitive bioluminescence assay for drug screening on Leishmania.
Paloque, Lucie; Vidal, Nicolas; Casanova, Magali; Dumètre, Aurélien; Verhaeghe, Pierre; Parzy, Daniel; Azas, Nadine.
Affiliation
  • Paloque L; Infections Parasitaires, Transmission, Physiopathologie et Thérapeutique, UMR MD3, Faculté de Pharmacie, Aix-Marseille Université, 27 Boulevard Jean Moulin, CS 30064, 13385 Marseille Cedex 05, France. Electronic address: lupaloque@hotmail.com.
J Microbiol Methods ; 95(3): 320-3, 2013 Dec.
Article in En | MEDLINE | ID: mdl-24055386
We validated a new method, based on luciferine/luciferase bioluminescence, for drug screening on promastigotes of different Leishmania species. Results obtained with this new, rapid, reproducible, and reliable method are in good accordance with results obtained by the conventional MTT assay. This bioluminescence assay has a lower detection limit.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drug Evaluation, Preclinical / Leishmania / Luminescent Measurements / Antiprotozoal Agents Type of study: Diagnostic_studies / Evaluation_studies / Screening_studies Language: En Journal: J Microbiol Methods Year: 2013 Document type: Article Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drug Evaluation, Preclinical / Leishmania / Luminescent Measurements / Antiprotozoal Agents Type of study: Diagnostic_studies / Evaluation_studies / Screening_studies Language: En Journal: J Microbiol Methods Year: 2013 Document type: Article Country of publication: