Predictive observation-based endpoint criteria for mice receiving total body irradiation.
Comp Med
; 63(4): 313-22, 2013 Aug.
Article
in En
| MEDLINE
| ID: mdl-24209966
ABSTRACT
Total body irradiation of mice is a commonly used research technique; however, humane endpoints have not been clearly identified. This situation has led to the inconsistent use of various endpoints, including death. To address this issue, we refined a cageside observation-based scoring system specifically for mice receiving total body irradiated. Male and female C57BL/6 mice (age, 8 wk) received 1 of 3 doses of radiation from 1 of 2 different radiation sources and were observed for progression of clinical signs. All mice were scored individually by using cageside observations of their body posture (score, 0 to 3), eye appearance (0 to 3), and activity level (0 to 3). Retrospective analysis of the observation score data indicated that death could be predicted accurately with total scores of 7 or greater, and observation scores were consistent between observers. This scoring system can be used to increase the consistent use of endpoint criteria in total body murine irradiation studies and ultimately to improve animal welfare.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rodent Diseases
/
Animal Welfare
/
Whole-Body Irradiation
/
Acute Radiation Syndrome
Type of study:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Comp Med
Journal subject:
MEDICINA
/
MEDICINA VETERINARIA
Year:
2013
Document type:
Article
Affiliation country: