Endomembrane and Golgi traffic in plant cells.

ABSTRACT

Leaf epidermal cells make ideal specimens for the investigation of the plant secretory pathway in that it is relatively easy to tag with fluorescent proteins and visualize in vivo the various organelles of the pathway. A number of techniques can be employed to identify and study proteins within the endomembrane organelles and to study their dynamics and interactions. Here, we discuss the most commonly used approaches to express proteins within arabidopsis and tobacco leaves, the use of mutant screens to identify trafficking proteins, and the use of two in vivo techniques, Fluorescence recovery after photobleaching and Förster resonance energy transfer, to study protein dynamics in plant cells.