Endomembrane and Golgi traffic in plant cells.
Methods Cell Biol
; 118: 69-83, 2013.
Article
in En
| MEDLINE
| ID: mdl-24295301
ABSTRACT
Leaf epidermal cells make ideal specimens for the investigation of the plant secretory pathway in that it is relatively easy to tag with fluorescent proteins and visualize in vivo the various organelles of the pathway. A number of techniques can be employed to identify and study proteins within the endomembrane organelles and to study their dynamics and interactions. Here, we discuss the most commonly used approaches to express proteins within arabidopsis and tobacco leaves, the use of mutant screens to identify trafficking proteins, and the use of two in vivo techniques, Fluorescence recovery after photobleaching and Förster resonance energy transfer, to study protein dynamics in plant cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nicotiana
/
Arabidopsis
/
Plant Leaves
/
Golgi Apparatus
/
Intracellular Membranes
Language:
En
Journal:
Methods Cell Biol
Year:
2013
Document type:
Article
Affiliation country: