miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes.
Virology
; 448: 210-6, 2014 Jan 05.
Article
in En
| MEDLINE
| ID: mdl-24314651
ABSTRACT
A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Repressor Proteins
/
Keratinocytes
/
Oncogene Proteins, Viral
/
Papillomavirus Infections
/
MicroRNAs
/
Human papillomavirus 16
/
Papillomavirus E7 Proteins
Type of study:
Prognostic_studies
Limits:
Humans
/
Male
Language:
En
Journal:
Virology
Year:
2014
Document type:
Article