Quantification of capillary blood cell flow using reflectance confocal microscopy.
Skin Res Technol
; 20(3): 373-8, 2014 Aug.
Article
in En
| MEDLINE
| ID: mdl-24506277
ABSTRACT
BACKGROUND/PURPOSE:
In vivo reflectance confocal microscopy (IVCM) is a new tool for skin microcirculation. However, the measure of quantitative blood cell flow (QBCF) has not been standardized. We studied the inter-investigator and the intra-capillary reproducibility of the manual measure of QBCF on IVCM videos and investigated if a software program might help measure QBCF and be sensitive to vascular occlusion tests.METHODS:
The inter-investigator reproducibility of the manual QBCF was evaluated on 107 videos. The intra-capillary reproducibility of QBCF measured manually and by 2 semi-automatic procedures based on Image J software analysis was evaluated on 19 capillaries. One of the semi-automatic methods (peaks of luminous intensity) was also used to measure the QBCF during vascular occlusion tests.RESULTS:
The manual measure did not show a good inter-investigator reproducibility (Pearson's coefficient <0.5). The 'peaks of luminous intensity' method was found to have a good intra-capillary reproducibility and to be sensitive to vascular occlusion.CONCLUSION:
Differently from the manual count, the count of peaks of luminous intensity by Image J software seems to be promising to measure QBCF. The future is to create software allowing for real-time measure of the QBCF based on the peaks of luminous intensity inside the capillaries recorded by IVCM.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rheology
/
Blood Flow Velocity
/
Capillaries
/
Image Interpretation, Computer-Assisted
/
Microscopy, Confocal
/
Microscopy, Interference
Type of study:
Diagnostic_studies
Limits:
Adult
/
Humans
/
Male
Language:
En
Journal:
Skin Res Technol
Journal subject:
DERMATOLOGIA
Year:
2014
Document type:
Article
Affiliation country: