Vasonatrin peptide stimulates both of the natriuretic peptide receptors, NPRA and NPRB.
Biochem Biophys Res Commun
; 446(4): 1276-80, 2014 Apr 18.
Article
in En
| MEDLINE
| ID: mdl-24699414
ABSTRACT
Vasonatrin peptide (VNP) is an active cardiovascular factor and a novel synthetic natriuretic peptide with unknown natriuretic peptide receptor (NPR) binding properties. We set out to design binding models of NPRA/VNP and NPRB/VNP, and then assessed their recognition and binding affinities using molecular dynamics. Molecular dynamics analysis indicated decreases in the values of Van der Waals, electrostatic energy and potential energy of NPRB/VNP compared to NPRA/VNP. There was a 25% increase in H-bond formation between VNP and NPRB. The cGMP stimulated by VNP in NPRB-transfected HEK-293 cells was 11-fold higher than that of NPRA. We therefore demonstrated that VNP binds with both NPRA and NPRB, but with a preference for NPRB.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Atrial Natriuretic Factor
/
Receptors, Atrial Natriuretic Factor
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2014
Document type:
Article