Chromatographic analysis of the flavonoids from robinia pseudoacacia species.

ABSTRACT

THE PURPOSE OF THIS STUDY WAS THE QUALITATIVE AND QUANTITATIVE ANALYSIS OF FLAVONOIDS FROM ROBINIA PSEUDOACACIA USING TWO DIFFERENT TECHNIQUES OF ANALYSIS: Thin Layer Chromatography (TLC) and TLC coupled with photo-densitometry. The results obtained by chromatographic analysis showed a higher concentration of flavonoids in flowers than in leaves. The flowers harvested in the plains have a higher concentration of hyperoside (0.9 mg/mL) compared with the flowers collected from the hills (0.54 mg/mL). The leaves are richer in ruthoside (0.98 mg/mL) compared with the flowers.