In vitro selection of DNA-based aptamers that exhibit RNA-like conformations using a chimeric oligonucleotide library that contains two different xeno-nucleic acids.
Mol Biosyst
; 11(1): 71-6, 2015 Jan.
Article
in En
| MEDLINE
| ID: mdl-25325213
ABSTRACT
We successfully generated chimeric DNA aptamers that contained six nucleoside analogs of 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) in the primer region and multiple guanosine analogs of 2'-deoxy-2'-fluoro-ribonucleic acid (FNA) in the non-primer region using capillary electrophoresis-systematic evolution of ligands by exponential enrichment (CE-SELEX). Active species enrichment became saturated only after five selection rounds, and we obtained DNA-based xeno-nucleic acid (XNA) aptamers that had high binding affinities for the target human thrombin, with dissociation constant (Kd) values of ≥10 nanomolar. Based on sequence and circular dichroism (CD) analyses, these XNA aptamers exhibited RNA-like conformations, which could cause DNA-based strands to adopt structurally diverse conformations.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
RNA
/
Nucleic Acids
/
Gene Library
/
Aptamers, Nucleotide
/
SELEX Aptamer Technique
Limits:
Humans
Language:
En
Journal:
Mol Biosyst
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2015
Document type:
Article
Affiliation country: