Start-up of low-temperature anammox in UASB from mesophilic yeast factory anaerobic tank inoculum.
Environ Technol
; 36(1-4): 214-25, 2015.
Article
in En
| MEDLINE
| ID: mdl-25413116
ABSTRACT
Robust start-up of the anaerobic ammonium oxidation (anammox) process from non-anammox-specific seeding material was achieved by using an inoculation with sludge-treating industrial [Formula see text]-, organics- and N-rich yeast factory wastewater. N-rich reject water was treated at 20°C, which is significantly lower than optimum treatment temperature. Increasing the frequency of biomass fluidization (from 1-2 times per day to 4-5 times per day) through feeding the reactor with higher flow rate resulted in an improved total nitrogen removal rate (from 100 to 500 g m(-3)d(-1)) and increased anammox bacteria activity. As a result of polymerase chain reaction (PCR) tests, uncultured planctomycetes clone 07260064(4)-2-M13-_A01 (GenBank JX852965) was identified from the biomass taken from the reactor. The presence of anammox bacteria after cultivation in the reactor was confirmed by quantitative PCR (qPCR); an increase in quantity up to â¼2×10(6) copies g VSS(-1) during operation could be seen in qPCR. Statistical modelling of chemical parameters revealed the roles of several optimized parameters needed for a stable process.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sewage
/
Yeasts
/
Bioreactors
/
Culture Media
/
Ammonium Compounds
Type of study:
Prognostic_studies
Language:
En
Journal:
Environ Technol
Journal subject:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Year:
2015
Document type:
Article
Affiliation country: