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Stimulation-evoked Ca2+ signals in astrocytic processes at hippocampal CA3-CA1 synapses of adult mice are modulated by glutamate and ATP.
Tang, Wannan; Szokol, Karolina; Jensen, Vidar; Enger, Rune; Trivedi, Chintan A; Hvalby, Øivind; Helm, P Johannes; Looger, Loren L; Sprengel, Rolf; Nagelhus, Erlend A.
Affiliation
  • Tang W; Centre for Molecular Medicine Norway, The Nordic EMBL Partnership, Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway, Department of Molecular Neurobiology and wannan.tang@medisin.uio.no e.a.nagelhus@medisin.uio.no.
  • Szokol K; Centre for Molecular Medicine Norway, The Nordic EMBL Partnership, Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway, Oslo University Hospital, Department of Neurology, 0027 Oslo, Norway.
  • Jensen V; Centre for Molecular Medicine Norway, The Nordic EMBL Partnership, Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway.
  • Enger R; Centre for Molecular Medicine Norway, The Nordic EMBL Partnership, Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway, Oslo University Hospital, Department of Neurology, 0027 Oslo, Norway.
  • Trivedi CA; Neural Circuits and Behaviour Research Group, Department of Biomedical Optics, Max Planck Institute for Medical Research, D-69120 Heidelberg, Germany, and.
  • Hvalby Ø; Department of Physiology, and.
  • Helm PJ; Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway.
  • Looger LL; Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia 20147, and.
  • Sprengel R; Department of Molecular Neurobiology and.
  • Nagelhus EA; Centre for Molecular Medicine Norway, The Nordic EMBL Partnership, Letten Centre and GliaLab, Department of Physiology, Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo, Norway, Oslo University Hospital, Department of Neurology, 0027 Oslo, Norway, Center for Translational Neuromedi
J Neurosci ; 35(7): 3016-21, 2015 Feb 18.
Article in En | MEDLINE | ID: mdl-25698739
To date, it has been difficult to reveal physiological Ca(2+) events occurring within the fine astrocytic processes of mature animals. The objective of the study was to explore whether neuronal activity evokes astrocytic Ca(2+) signals at glutamatergic synapses of adult mice. We stimulated the Schaffer collateral/commissural fibers in acute hippocampal slices from adult mice transduced with the genetically encoded Ca(2+) indicator GCaMP5E driven by the glial fibrillary acidic protein promoter. Two-photon imaging revealed global stimulation-evoked astrocytic Ca(2+) signals with distinct latencies, rise rates, and amplitudes in fine processes and somata. Specifically, the Ca(2+) signals in the processes were faster and of higher amplitude than those in the somata. A combination of P2 purinergic and group I/II metabotropic glutamate receptor (mGluR) antagonists reduced the amplitude of the Ca(2+) transients by 30-40% in both astrocytic compartments. Blockage of the mGluRs alone only modestly reduced the magnitude of the stimulation-evoked Ca(2+) signals in processes and failed to affect the somatic Ca(2+) response. Local application of group I or I/II mGluR agonists or adenosine triphosphate (ATP) elicited global astrocytic Ca(2+) signals that mimicked the stimulation-evoked astrocytic Ca(2+) responses. We conclude that stimulation-evoked Ca(2+) signals in astrocytic processes at CA3-CA1 synapses of adult mice (1) differ from those in astrocytic somata and (2) are modulated by glutamate and ATP.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Synapses / Adenosine Triphosphate / Astrocytes / Glutamic Acid / Calcium Signaling / Hippocampus Limits: Animals / Humans Language: En Journal: J Neurosci Year: 2015 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Synapses / Adenosine Triphosphate / Astrocytes / Glutamic Acid / Calcium Signaling / Hippocampus Limits: Animals / Humans Language: En Journal: J Neurosci Year: 2015 Document type: Article Country of publication: