Label-free quantitative mass spectrometry for analysis of protein antigens in a meningococcal group B outer membrane vesicle vaccine.
Hum Vaccin Immunother
; 11(6): 1518-25, 2015.
Article
in En
| MEDLINE
| ID: mdl-25997113
Key words
BCA, bicinchoninic acid; ELISA, enzyme-linked immunosorbent assay; HPLC high performance liquid chromatography; LC-MS/MS, liquid chromatography, tandem mass spectrometry; MRM, multiple reaction monitoring; MS, mass spectrometry; MenB, Meningococcal serogroup B; NadA, Neisseria adhesin A; OMPC, outer membrane protein complex; OMV, outer membrane vesicle; PorA, major outer membrane protein (class I outer membrane protein); QC, quality control; RSD, relative standard deviation; SDS-PAGE, sodium dodecyl sulfate, polyacrylamide gel electrophoresis; WRAIR, Walter Reed Army Institute of Research; fHbp, factor H binding protein; mass spectrometry; membrane proteins; meningococcal group B; multiple reaction monitoring; outer membrane vesicle; protein quantification; vaccine
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Chromatography, Liquid
/
Meningococcal Vaccines
/
Tandem Mass Spectrometry
/
Antigens, Bacterial
Limits:
Humans
Language:
En
Journal:
Hum Vaccin Immunother
Year:
2015
Document type:
Article
Affiliation country:
Country of publication: