Effects of Wnt-10b on proliferation and differentiation of murine melanoma cells.
Biochem Biophys Res Commun
; 463(4): 618-23, 2015 Aug 07.
Article
in En
| MEDLINE
| ID: mdl-26056007
ABSTRACT
In spite of the strong expression of Wnt-10b in melanomas, its role in melanoma cells has not been elucidated. In the present study, the biological effects of Wnt-10b on murine B16F10 (B16) melanoma cells were investigated using conditioned medium from Wnt-10b-producing COS cells (Wnt-CM). After 2 days of culture in the presence of Wnt-CM, proliferation of B16 melanoma cells was inhibited, whereas tyrosinase activity was increased. An in vitro wound healing assay demonstrated that migration of melanoma cells to the wound area was inhibited with the addition of Wnt-CM. Furthermore, evaluation of cellular senescence revealed prominent induction of SA-ß-gal-positive senescent cells in cultures with Wnt-CM. Finally, the growth of B16 melanoma cell aggregates in collagen 3D-gel cultures was markedly suppressed in the presence of Wnt-CM. These results suggest that Wnt-10b represses tumor cell properties, such as proliferation and migration of B16 melanoma cells, driving them toward a more differentiated state along a melanocyte lineage.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Melanoma, Experimental
/
Cell Differentiation
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Cell Proliferation
/
Wnt Proteins
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2015
Document type:
Article
Affiliation country: