Autocrine motility factor receptor promotes the proliferation of human acute monocytic leukemia THP-1 cells.
Int J Mol Med
; 36(3): 627-32, 2015 Sep.
Article
in En
| MEDLINE
| ID: mdl-26136223
ABSTRACT
The aberrant activation of autocrine motility factor receptor (AMFR) has been implicated in several types of human cancer. The present study aimed to elucidate the effect of AMFR on the regulation of proliferation in an acute monocytic leukemia cell line, THP1. THP1 cells were transfected with AMFRtargeted small interfering (si)RNA and a plasmid encoding a truncated AMFR, AMFRC, (pcDNA3.1AMFRC). The mRNA and protein levels of AMFR and the downstream targets, rhoassociated, coiledcoil containing protein kinase 2 (ROCK2), cyclin D1, and Bcell lymphoma (Bcl)2, were measured using reverse transcriptionquantitatibe polymerase chain reaction and immunoblot analyses. The effects on cell cycle and apoptosis were investigated using flow cytometry. The present study successfully established the knockdown of AMFR and expression of AMFRC in the THP1 cells. Downregulation of AMFR induced cell cycle arrest at the G0/G1 phase, and increased apoptosis of the THP1 cells (all P<0.05). The AMFR siRNA increased the percentage of early apoptotic cells between 3.88±1.43 and 19.58±4.29% (P<0.05). The expression levels of ROCK2, cyclin D1 and Bcl2 were reduced by the downregulation of AMFR and enhanced by overexpression of AMFRC. In conclusion, AMFR appears to be crucial for the proliferation of the THP1 acute monocytic leukemia cell line. Therefore, AMFR may represent a potential target for the treatment of acute monocytic leukemia.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Leukemia, Monocytic, Acute
/
Cell Proliferation
/
Receptors, Autocrine Motility Factor
Limits:
Humans
Language:
En
Journal:
Int J Mol Med
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Year:
2015
Document type:
Article