Recording axonal conduction to evaluate the integration of pluripotent cell-derived neurons into a neuronal network.
Biomed Microdevices
; 17(5): 94, 2015 Oct.
Article
in En
| MEDLINE
| ID: mdl-26303583
ABSTRACT
Stem cell transplantation is a promising therapy to treat neurodegenerative disorders, and a number of in vitro models have been developed for studying interactions between grafted neurons and the host neuronal network to promote drug discovery. However, methods capable of evaluating the process by which stem cells integrate into the host neuronal network are lacking. In this study, we applied an axonal conduction-based analysis to a co-culture study of primary and differentiated neurons. Mouse cortical neurons and neuronal cells differentiated from P19 embryonal carcinoma cells, a model for early neural differentiation of pluripotent stem cells, were co-cultured in a microfabricated device. The somata of these cells were separated by the co-culture device, but their axons were able to elongate through microtunnels and then form synaptic contacts. Propagating action potentials were recorded from these axons by microelectrodes embedded at the bottom of the microtunnels and sorted into clusters representing individual axons. While the number of axons of cortical neurons increased until 14 days in vitro and then decreased, those of P19 neurons increased throughout the culture period. Network burst analysis showed that P19 neurons participated in approximately 80% of the bursting activity after 14 days in vitro. Interestingly, the axonal conduction delay of P19 neurons was significantly greater than that of cortical neurons, suggesting that there are some physiological differences in their axons. These results suggest that our method is feasible to evaluate the process by which stem cell-derived neurons integrate into a host neuronal network.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Action Potentials
/
Pluripotent Stem Cells
/
Neural Stem Cells
/
Nerve Net
/
Neural Conduction
/
Neurons
Limits:
Animals
Language:
En
Journal:
Biomed Microdevices
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2015
Document type:
Article