De novo transcriptome reconstruction and annotation of the Egyptian rousette bat.
BMC Genomics
; 16: 1033, 2015 Dec 07.
Article
in En
| MEDLINE
| ID: mdl-26643810
ABSTRACT
BACKGROUND:
The Egyptian Rousette bat (Rousettus aegyptiacus), a common fruit bat species found throughout Africa and the Middle East, was recently identified as a natural reservoir host of Marburg virus. With Ebola virus, Marburg virus is a member of the family Filoviridae that causes severe hemorrhagic fever disease in humans and nonhuman primates, but results in little to no pathological consequences in bats. Understanding host-pathogen interactions within reservoir host species and how it differs from hosts that experience severe disease is an important aspect of evaluating viral pathogenesis and developing novel therapeutics and methods of prevention.RESULTS:
Progress in studying bat reservoir host responses to virus infection is hampered by the lack of host-specific reagents required for immunological studies. In order to establish a basis for the design of reagents, we sequenced, assembled, and annotated the R. aegyptiacus transcriptome. We performed de novo transcriptome assembly using deep RNA sequencing data from 11 distinct tissues from one male and one female bat. We observed high similarity between this transcriptome and those available from other bat species. Gene expression analysis demonstrated clustering of expression profiles by tissue, where we also identified enrichment of tissue-specific gene ontology terms. In addition, we identified and experimentally validated the expression of novel coding transcripts that may be specific to this species.CONCLUSION:
We comprehensively characterized the R. aegyptiacus transcriptome de novo. This transcriptome will be an important resource for understanding bat immunology, physiology, disease pathogenesis, and virus transmission.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chiroptera
/
Computational Biology
/
Molecular Sequence Annotation
/
Transcriptome
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
BMC Genomics
Journal subject:
GENETICA
Year:
2015
Document type:
Article
Affiliation country: