Highly sensitive and selective fluorescent assay for guanine based on the Cu(2+)/eosin Y system.
Spectrochim Acta A Mol Biomol Spectrosc
; 161: 150-4, 2016 May 15.
Article
in En
| MEDLINE
| ID: mdl-26971024
ABSTRACT
A fluorescent probe has been developed for the determination of guanine based on the quenched fluorescence signal of Cu(2+)/eosin Y. Cu(2+) interacted with eosin Y, resulting in fluorescence quenching. Subsequently, with the addition of guanine to the Cu(2+)/eosin Y system, guanine reacted with Cu(2+) to form 11 chelate cation, which further combined with eosin Y to form a 11 ternary ion-association complex by electrostatic attraction and hydrophobic interaction, resulting in significant decrease of the fluorescence. Hence, a fluorescent system was constructed for rapid, sensitive and selective detection of guanine with a detection limit as low as 1.5 nmol L(-1) and a linear range of 3.3-116 nmol L(-1). The method has been applied satisfactorily to the determination of guanine in DNA and urine samples with the recoveries from 98.7% to 105%. This study significantly expands the realm of application of ternary ion-association complex in fluorescence probe.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Eosine Yellowish-(YS)
/
Fluorescent Dyes
/
Guanine
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Journal subject:
BIOLOGIA MOLECULAR
Year:
2016
Document type:
Article
Affiliation country: