Myomir dysregulation and reactive oxygen species in aged human satellite cells.
Biochem Biophys Res Commun
; 473(2): 462-70, 2016 Apr 29.
Article
in En
| MEDLINE
| ID: mdl-26975470
ABSTRACT
Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aging
/
Gene Expression Regulation
/
Reactive Oxygen Species
/
Muscle Development
/
Satellite Cells, Skeletal Muscle
/
MicroRNAs
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Humans
/
Male
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2016
Document type:
Article