Your browser doesn't support javascript.
loading
VY6, a ß-lactoglobulin-derived peptide, altered metabolic lipid pathways in the zebra fish liver.
Mohammed-Geba, K; Arrutia, F; Do-Huu, H; Borrell, Y J; Galal-Khallaf, A; Ardura, A; Riera, Francisco A; Garcia-Vazquez, Eva.
Affiliation
  • Mohammed-Geba K; Department of Functional Biology, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain. egv@uniovi.es and Genetic Engineering and Molecular Biology Division, Faculty of Science, Menoufia University, Egypt.
  • Arrutia F; Department of Chemical Engineering and Environmental Technology, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.
  • Do-Huu H; Department of Aquaculture Biotechnology, Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da, Nha Trang, Vietnam.
  • Borrell YJ; Department of Functional Biology, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain. egv@uniovi.es.
  • Galal-Khallaf A; Department of Functional Biology, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain. egv@uniovi.es and Genetic Engineering and Molecular Biology Division, Faculty of Science, Menoufia University, Egypt.
  • Ardura A; Department of Functional Biology, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain. egv@uniovi.es.
  • Riera FA; Department of Chemical Engineering and Environmental Technology, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.
  • Garcia-Vazquez E; Department of Functional Biology, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain. egv@uniovi.es.
Food Funct ; 7(4): 1968-74, 2016 Apr.
Article in En | MEDLINE | ID: mdl-26983953
Today enormous research efforts are being focused on alleviating the massive, adverse effects of obesity. Short peptides are key targets for research as they can be generated from natural proteins, like milk. Here we conducted trypsinogen digestion of beta-lactoglobulin (ß-lg), the major mammalian milk protein, to release the hexamer VY6. It was assayed in vivo for its activities on lipid metabolism using zebra fish as a vertebrate model. Zebra fish juveniles were injected with two different doses of the peptide: 100 and 800 µg per g fish and left for 5 days before sacrificing. Lipid measurements showed significant reduction in liver triglycerides and free cholesterol, as well as increased liver HDL cholesterol. Dose-dependent increases of the mRNA levels of the genes coding for the enzymes acyl coenzyme A oxidase 1 (acox1) and lipoprotein lipase (lpl) were also found. The complete results suggest significant anti-obesity activity of the ß-lg-derived VY6 peptide. Its use as a nutraceutical has been discussed.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Zebrafish / Lactoglobulins / Liver / Obesity Type of study: Prognostic_studies Limits: Animals / Female / Humans / Male Language: En Journal: Food Funct Year: 2016 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Zebrafish / Lactoglobulins / Liver / Obesity Type of study: Prognostic_studies Limits: Animals / Female / Humans / Male Language: En Journal: Food Funct Year: 2016 Document type: Article Affiliation country: Country of publication: